Genomic Tales

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چکیده

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Repeated TALEs

Three recent papers, published just weeks apart, describe the use of fluorescent TALEs to tag specific DNA sequences in live cells and, in one case, also in fixed cells, the latter with potential clinical applications.

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Bacteriophage tales

A correspondent has asked me about the origins of a legend which has become known as 'The phage in the letter'. Common to all variants of this story is the following: one scientist, called X, sends a request to another, Z, for a particular bacteriophage strain which Z has discovered. Z replies saying he is not sending it out. X thereupon plates out the letter and retrieves the phage from it. So...

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Pathogenetic tales

A little over twenty years ago, a group of scientists assembled in Asilomar, California, to consider the consequences of the newly invented techniques of genetic engineering, or recombinant DNA, as it later came to be called. There was in place a moratorium recommended by scientists and which we, working for the UK's Medical Research Council, had been instructed to heed by the Secretary of the ...

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Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages

The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 ...

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AnnoTALE: bioinformatics tools for identification, annotation, and nomenclature of TALEs from Xanthomonas genomic sequences

Transcription activator-like effectors (TALEs) are virulence factors, produced by the bacterial plant-pathogen Xanthomonas, that function as gene activators inside plant cells. Although the contribution of individual TALEs to infectivity has been shown, the specific roles of most TALEs, and the overall TALE diversity in Xanthomonas spp. is not known. TALEs possess a highly repetitive DNA-bindin...

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ژورنال

عنوان ژورنال: Science

سال: 2006

ISSN: 0036-8075,1095-9203

DOI: 10.1126/science.313.5795.1907